Recovery of Collagen/Gelatin from Fish Waste with Carbon Dioxide as a Green Solvent: An Optimization and Characterization

Fish processing produces large amounts of fish waste. Instead disposing it, it is wiser to recover the valuable resource for high-value-added products. Our study proposed a process using carbon dioxide-acidified water as green solvent under supercritical conditions successfully collagen/gelatin from skin and bone striped catfish. The optimum extraction were obtained at 75 bar, 37 °C, 24 h. yields dry mass around 37% 8%, respectively. extracted products characterized by Fourier-transformed infrared spectroscopy functional groups, scanning electron microscopy evaluate morphology, sodium dodecyl-sulfate polyacrylamide gel electrophoresis protein pattern, UV–vis analysis measure absorption peak, thermal gravimetric determine denaturation temperature. results show viability method on an industrial scale. characteristics product promising potential being developed further in many applications such biomaterial engineering healthcare or natural polymer-based absorbent material efficient removal heavy metals wastewater.